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目的:优选鲜地黄的闪式提取工艺。方法:以梓醇及干膏得量为评价指标,选取提取时间、提取次数及溶剂用量为考察因素,通过单因素试验及正交试验优选闪式提取工艺。采用RP-HPLC测定指标成分含量。结果:最佳闪式提取工艺为加3倍量95%乙醇提取1次,提取时间3 min。结论:优选的工艺快速、合理。
Objective: To optimize the flash extraction of fresh radix rehmanniae. Methods: Taking catalpol and dry extract as evaluation indexes, the extraction time, the number of extraction and the amount of solvent were selected as the investigation factors. The flash extraction process was optimized by single factor test and orthogonal test. RP-HPLC determination of the content of the indicator components. Results: The best flash extraction process for 3 times the amount of 95% ethanol extraction 1 time, extraction time 3 min. Conclusion: The preferred process is fast and reasonable.