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首次提出了亚硝酸氧化司帕沙星的荧光反应测定司帕沙星的新方法 ,在酸性介质中 ,亚硝酸氧化司帕沙星继而与碘化钾反应生成了新的荧光物质 ,其荧光发射较司帕沙星强 1 3 4倍 ,激发和发射波长分别为 2 86nm和464nm,,当司帕沙星的浓度为 2× 1 0 - 8mo L L～ 1× 1 0 - 6mo L L时 ,工作曲线呈良好的线性关系 ,检出限为 1 .5×1 0 - 1 0 mo L L。据此建立了同步 -导数荧光光谱法测定尿液中司帕沙星的新方法 ,本文还对反应产物的荧光特性及有关反应机理进行了探讨。
For the first time, a new method was proposed for the determination of sparfloxacin by the fluorescence reaction of sparfloxacin oxidized by nitrous acid. In acid medium, sparfloxacin oxidized by nitrous acid then reacts with potassium iodide to generate new fluorescent substance, Pasha star strong 1 3 4 times, the excitation and emission wavelengths were 2 86nm and 464nm, when the sparfloxacin concentration of 2 × 10 8mo LL ~ 1 × 10 6mo LL, the working curve was good The linearity of the detection limit was 1.5 × 10 ~ 10 mo LL. A new method for determination of sparfloxacin in urine by synchronous-derivative fluorescence spectrometry was established. The fluorescence characteristics of the reaction product and the reaction mechanism were also discussed.