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本研究以古犬肾为实验材料,模拟肾移植的手术环境,用循环水冷却囊装置来阻止供肾置入过程中发生的第二次温缺血,并对其效果作了测定。研究结果表明,使用此装置在模拟的38±1℃的肾移植环境中,可以很容易地将供肾的温度控制在4±1℃达3h以上。对比研究发现,用此装置冷却的肾脏,其组织结构完全正常;而在没有冷却的肾脏发现有大量的肾小管上皮坏死。供肾在植入过程中的这个温度与其在冷贮存或连续低温灌流保存时的温度完全一致,从而消除了肾移植过程中供肾的第二次温缺血。循环水冷却囊还有体积小、重量轻、对手术操作影响小等优点。
In this study, the canine kidney was used as the experimental material to simulate the surgical environment of renal transplantation. The circulating water cooling device was used to prevent the second warm ischemia that occurred during the renal insufficiency. The effect was also determined. The results show that the use of this device in the simulated 38 ± 1 ℃ renal transplant environment, the donor can be easily controlled at 4 ± 1 ℃ for 3h or more. Comparative study found that with this device to cool the kidneys, its structure is completely normal; in the absence of cooling of the kidneys found a large number of tubular epithelial necrosis. This temperature of the donor kidney during implantation is exactly the same as the temperature at which it is preserved during cold storage or continuous hypothermic perfusion to eliminate the second warm ischemia of the kidneys during kidney transplantation. Circulating water cooling capsule there is small size, light weight, the advantages of small surgical operations.