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目的 探讨血管紧张素原 (AGT)基因 M2 35 T多态性与中国人高血压病合并脑梗塞的关系。方法 应用聚合酶链反应 -限制性片段长度多性态分析结合 DNA银染技术 ,对 83例正常人 ,83例高血压病无心脑血管合并症患者 ,5 5例高血压病合并脑梗塞患者进行 AGT基因 M2 35 T多态性分析。结果 AGT基因 M2 35 T多态性与中国人高血压病无相关关系 ,但有高血压家族史的脑梗塞患者 AGT基因 TT基因型频率 6 1 .8%和 T等位基因频率 76 .4%显著高于正常对照组的 42 .7%和 6 5 .2 % (P<0 .0 5 ) ,以及高血压病无心脑血管合并症组的 44 .6 %和 6 8.1 % (P<0 .0 5 )。结论 有高血压家族史的 AGT基因 TT基因型携带者易患脑梗塞 ,这种基因型可能是中国人有高血压家族史的脑梗塞发病的重要遗传因素。
Objective To investigate the relationship between the M235T polymorphism of angiotensinogen (AGT) gene and Chinese patients with hypertension and cerebral infarction. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and DNA-silver staining were used in 83 normal subjects, 83 hypertensive patients without cardiovascular and cerebrovascular complications, 55 hypertensive patients with cerebral infarction AGT gene M235 T polymorphism analysis. Results There was no association between AGT gene M235T polymorphism and Chinese hypertension, but the TT genotype frequency of AGT gene was 61.8% and T allele frequency was 76.4% in patients with a family history of hypertension. Were significantly higher than those in the control group (42.7% vs 65.2%, P <0.05), and were 44.6% and 61.1% (P <0) in patients with hypertension without cardiovascular and cerebrovascular complications .0 5). Conclusions AGT TT genotype carriers with family history of hypertension are predisposed to cerebral infarction. This genotype may be an important genetic factor for the onset of cerebral infarction in Chinese with a family history of hypertension.