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目的调查中华按蚊对DDT、溴氰菊酯、氟氯氰菊酯和马拉硫磷抗药性,为合理使用杀虫剂提供科学依据。方法采用WHO成蚊滤纸接触法,观察区分剂量击倒率、首只蚊虫击倒时间和死亡率,根据死亡率判定抗性级别。结果广水、京山、襄阳等地中华按蚊对DDT抗击倒力最强,区分剂量击倒率分别为4.86%和0,首只蚊虫击倒时间为24min和>60min,其次是溴氰菊酯和氟氯氰菊酯,击倒率分别为10.31、2.91、3.12%和15.00、4.90、2.16%,首只蚊虫击倒时间为19、25、32min和20、5、33min,。区分剂量死亡率:DDT、溴氰菊酯和氟氯氰菊酯均<50%,为高抗群体,马拉硫磷分别为58.66、10.20和25.23%,为抗性群体。结论中华按蚊对溴氰菊酯抗性发展迅猛,对DDT抗性居高不下,可能将成为疟疾媒介控制的最大障碍,应加强监测。
Objective To investigate the resistance of Anopheles sinensis to DDT, deltamethrin, cyfluthrin and malathion to provide a scientific basis for the rational use of insecticides. Methods WHO adult mosquito filter paper contact method was used to observe the dose-down rate, the first mosquito knock-down time and mortality, and the level of resistance was judged according to the mortality rate. Results Anopheles sinensis in Guangshui, Jingshan and Xiangyang had the strongest resistance to DDT, and the differentiated dose knockdown rates were 4.86% and 0 respectively. The first mosquito knockdown time was 24min and> 60min, followed by deltamethrin And cyfluthrin, knockdown rates were 10.31,2.91,3.12% and 15.00,4.90,2.16%, the first mosquito knockdown time was 19,25,32min and 20,5,33min. Dose-rate mortality: DDT, deltamethrin and cyfluthrin were all <50%, being highly resistant, with malathion 58.66, 10.20 and 25.23%, respectively, as resistant populations. Conclusion The development of deltamethrin resistance in Anopheles sinensis is rapid and the resistance to DDT is high, which may be the biggest obstacle to malaria vector control. Monitoring should be strengthened.