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介绍了一种新型的基于免疫磁珠分选法的外周血循环肿瘤细胞(circulate tumor cell,CTC)捕捉芯片的制作方法,使得芯片的磁性微柱和流道可以同步成型,简化了制作流程,弥补了传统电铸工艺难以稳定实现高深宽比结构的不足,适应性和扩展性都比较好。同时针对人结肠癌培养细胞,结合图像处理技术,对细胞荧光图像进行了细胞的自动分割和计数,实现了一个细胞捕获和分析自动化系统,从而极大地提高了CTC检测的效率。主要研究了细胞捕获芯片的制作和图像处理的方法等。结果表明该细胞捕获分析系统可以给出较好的细胞捕获和计数结果,从而显著提高工作效率。
A novel method for manufacturing a capture chip for peripheral blood circulation tumor cells (CTCs) based on immunomagnetic bead sorting is introduced, which makes it possible to synchronously shape the magnetic microcolumn and the flow channel of the chip, simplifying the manufacturing process and making up The traditional electroforming process is difficult to achieve the stability of high aspect ratio structure of the lack of adaptability and scalability are better. At the same time, according to the culture of human colon cancer cells, combined with image processing technology, cell fluorescence images were automatically segmented and counted cells to achieve a cell capture and analysis automation system, thus greatly improving the CTC detection efficiency. The main study of the cell capture chip production and image processing methods. The results show that the cell capture analysis system can give better cell capture and counting results, thereby significantly improving work efficiency.