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目的阐明模拟微重力因素对肺循环局部调节功能的影响。方法采用尾部悬吊大鼠模型模拟微重力效应 ,制备离体动脉环测定肺动脉对几种血管活性物质的反应性变化。结果与对照组相比 ,尾部悬吊 7d内皮完整组大鼠肺动脉对氯化钾 ( 1 0～ 70mmol/L)的收缩反应无显著性变化 ,对苯肾上腺素( 1 0 - 9～ 1 0 - 5 mol/L)的收缩反应明显降低 ,对乙酰胆碱 ( 1 0 - 9～ 1 0 - 5 mol/L)的舒张反应增强 ,对卡托普利 ( 1 0 - 5～ 3× 1 0 - 3 mol/L)的舒张反应无明显变化。尾部悬吊 7d去内皮组大鼠肺动脉对氯化钾、苯肾上腺素、硝普钠的反应性较对照组无明显变化。结论尾部悬吊 7d引起大鼠肺动脉内皮细胞释放一氧化氮 (NO)增加 ,肺动脉舒张功能增强。
Objective To elucidate the effect of simulated microgravity factors on the local regulation of pulmonary circulation. Methods The tail-suspended rat model was used to simulate the effects of microgravity, and the isolated arterial rings were prepared for the determination of pulmonary artery reactivity to several vasoactive substances. Results Compared with the control group, there was no significant change in the pulmonary artery contractions in the intact endothelium of rats on the 7th day after tail suspension. The contractile response to potassium chloride (10 ~ 70mmol / L) 5 mol / L) significantly decreased the contractile response to acetylcholine (10 - 9 ~ 10 ~ 5 mol / L) and increased the diastolic response to captopril (10 ~ 5 ~ 3 × 10 ~ 3 mol / L) no significant change in the relaxation response. The reactivity of pulmonary arteries of rats in the endothelium group to tail suspension 7d did not change significantly to potassium chloride, phenylephrine and sodium nitroprusside compared with the control group. Conclusion The tail suspension for 7 days caused the release of nitric oxide (NO) in pulmonary artery endothelial cells and the enhancement of pulmonary artery diastolic function.