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目的建立全血中氯丙嗪的UPLC-MS/MS分析方法。方法采用乙腈沉淀蛋白,以Waters ACQUITY UPLCBEH C18柱(2.1mm×50mm,1.7μm)分离,乙腈-0.1%甲酸水溶液为流动相,梯度洗脱,正离子方式检测,多反应离子监测模式(MRM)。结果血液中氯丙嗪在1～100ng/mL范围内线形关系良好,检出限为0.01ng/mL,回收率为81.81%～89.36%,日内、日间精密度分别为9.3%、12.1%。结论本方法准确、快速,可用于全血中氯丙嗪的定性定量分析。
Objective To establish a UPLC-MS / MS method for the determination of chlorpromazine in whole blood. Methods The protein was separated by acetonitrile and separated on a Waters ACQUITY UPLC BEC C18 column (2.1 mm × 50 mm, 1.7 μm). The mobile phase consisted of acetonitrile - 0.1% formic acid as mobile phase, gradient elution, positive ion mode and multiple reaction mode (MRM) . Results The calibration curve of chlorpromazine in the blood ranged from 1 ng / mL to 100 ng / mL. The detection limit was 0.01 ng / mL and the recovery was 81.81% -89.36%. The intra-day and inter-day precision was 9.3% and 12.1% respectively. Conclusion The method is accurate and rapid and can be used for qualitative and quantitative analysis of chlorpromazine in whole blood.