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目的通过对巨结肠小肠结肠炎、无肠炎患儿及正常儿童大便中双歧杆菌和乳酸杆菌进行检测,初步探讨乳酸菌群和先天性巨结肠小肠结肠炎的关系。方法选取2006年1月至2006年12月在上海交通大学医学院附属新华医院行先天性巨结肠根治术的患儿共30例,1个月～7岁,平均年龄1.2岁。根据术前术后有无小肠结肠炎临床症状分为HD小肠结肠炎组和HD非肠炎组,其中肠炎10例。并选同龄儿10例为正常对照组。分别采集各组的新鲜大便,立即置入无菌离心管,-20℃冻存,24 h内抽提粪便内细菌基因组DNA。结果由于粪便内双歧杆菌和乳酸杆菌绝对值不呈正态分布,故取其对数值(log_(10))进行方差分析。各组双歧杆菌对数含量:巨结肠肠炎组7.07±0.85和巨结肠非肠炎组8.36±0.80、正常对照组8.46±0.92比较,差异均有统计学意义(P<0.05)。巨结肠非肠炎组和正常对照组相似,差异无统计学意义(P>0.05)。各组乳酸杆菌对数含量:巨结肠肠炎组5.51±0.65、巨结肠非肠炎组5.79±0.82,分别和正常对照组6.47±0.71比较,差异有统计学意义(P<0.05)。结论结合本实验结果提示在巨结肠患儿中双歧杆菌和乳酸杆菌的含量明显低于正常组,这可能是肠炎发生的原因之一。
Objective To investigate the relationship between lactic acid bacteria group and Hirschsprung’s disease by detecting Bifidobacterium and Lactobacillus in feces of patients with megacolour enterocolitis and non-colitis and normal children. Methods A total of 30 children with Hirschsprung’s disease from January 2006 to December 2006 in Xinhua Hospital affiliated to Shanghai Jiaotong University School of Medicine were selected, ranging from 1 month to 7 years with a mean age of 1.2 years. According to the clinical symptoms of preoperative and postoperative enterocolitis were divided into HD enterocolitis group and HD group, including enteritis in 10 cases. Ten children of same age were selected as normal control group. Fresh stools of each group were collected, immediately placed in sterile centrifuge tubes, frozen at -20 ℃, 24 h within the extraction of bacterial genomic DNA in the stool. Results As the absolute value of Bifidobacterium and Lactobacillus in the feces was not normally distributed, the logarithm value (log_ (10)) was analyzed by ANOVA. The logarithm content of Bifidobacterium in each group was 7.07 ± 0.85 in Hypertrophic enteritis group and 8.36 ± 0.80 in Hypertrophic enteritis group and 8.46 ± 0.92 in control group (all P <0.05). Hypertrophic enterocolitis group and the normal control group similar, the difference was not statistically significant (P> 0.05). The logarithm content of Lactobacilli in each group was 5.51 ± 0.65 in Hypertrophic enteritis group and 5.79 ± 0.82 in Hypertrophic enterocolitis group, which was significantly different from that in normal control group (6.47 ± 0.71, P <0.05). Conclusions The results of this experiment suggest that the content of Bifidobacterium and Lactobacillus in children with Hirschsprung ’s disease is significantly lower than that in the normal group, which may be one of the causes of enteritis.