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目的:探讨丹参对糖尿病大鼠肾脏的保护作用及其机制。方法:采用单侧肾切除、腹腔注射链脲佐菌素诱导糖尿病肾病大鼠模型,予以丹参药物干预。观察大鼠肾脏形态学及肾功能的变化。采用荧光实时定量逆转录-聚合酶链反应法检测转化生长因子β1(transforming growth factor-beta1,TGF-β1)、结缔组织生长因子(connective tissue growth factor,CTGF)、纤溶酶原激活物抑制物1(plasminogen activator inhibi-tor-1,PAI-1)等细胞因子在糖尿病大鼠肾皮质中的表达水平。结果:与正常对照组比较,用药第8周末糖尿病肾病模型组大鼠的肾脏肥大指数、平均肾小球体积、尿白蛋白排泄率、肌酐清除率均有明显升高(P<0.05),肾皮质TGF-β1、CTGF、PAI-1和纤维连接蛋白的表达水平也有显著增高(P<0.05)。丹参治疗组大鼠的上述指标与糖尿病肾病模型组比较,则有明显的降低(P<0.05)。结论:丹参可通过抑制TGF-β1、CTGF、PAI-1等细胞因子的表达,从而对糖尿病大鼠肾脏起保护作用。
Objective: To investigate the protective effect of salvia miltiorrhiza on the kidney of diabetic rats and its mechanism. METHODS: Rats with diabetic nephropathy were induced by unilateral nephrectomy and intraperitoneal injection of streptozotocin, and were treated with Salvia miltiorrhiza. Observe the change of kidney morphology and renal function in rats. Detection of transforming growth factor-β1 (TGF-β1), connective tissue growth factor (CTGF) and plasminogen activator inhibitor by fluorescence real-time quantitative reverse transcription-polymerase chain reaction The expression of cytokines such as plasmatic activator inhibitor-1 (PAI-1) in the renal cortex of diabetic rats. RESULTS: Compared with the normal control group, the kidney hypertrophy index, mean glomerular volume, urinary albumin excretion rate, and creatinine clearance rate in the diabetic nephropathy model group at the 8th week were all significantly increased (P<0.05). The expression levels of cortical TGF-β1, CTGF, PAI-1 and fibronectin were also significantly increased (P<0.05). Compared with the diabetic nephropathy model group, the above indexes of the Salvia miltiorrhiza treatment group were significantly decreased (P<0.05). Conclusion: Salvia miltiorrhiza can protect the kidney of diabetic rats by inhibiting the expression of cytokines such as TGF-β1, CTGF and PAI-1.