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目的研究妊娠期高血压疾病患者血清及胎盘组织中Endoglin水平的变化及临床意义。方法采用酶联免疫吸附双抗体夹心法(ELISA)及免疫组化方法检测68例妊娠期高血压疾病患者(妊娠期高血压疾病组,其中妊娠期高血压23例、轻度子痫前期22例、重度子痫前期23例)及20例正常妊娠妇女(对照组)血清及胎盘组织中Endoglin水平。结果妊娠期高血压疾病组血清可溶性Endoglin水平(29.66±8.52 ng/ml)明显高于对照组(10.42±4.35ng/ml),(P<0.01)。妊娠期高血压、轻度子痫前期、重度子痫前期患者血清中可溶性Endoglin水平逐渐升高(分别为13.24±6.17ng/ml,28.28±10.19ng/ml,42.50±13.48ng/ml),各组间差异有显著性(均P<0.01)。妊娠期高血压疾病组与对照组比较胎盘组织中Endoglin表达明显升高(P<0.01)。妊娠期高血压、轻、重度子痫前期胎盘组织中Endoglin表达逐渐增强,各组间差异具有显著性(P<0.05)。两组血清中可溶性Endoglin水平与胎盘组织Endoglin表达呈正相关(r=0.504,P<0.05及r=0.532,P<0.05)。结论 Endoglin水平升高可能与妊娠期高血压疾病的发病及病情发展有关。
Objective To study the changes of endoglin levels in serum and placenta of patients with gestational hypertension and its clinical significance. Methods Sixty-eight patients with gestational hypertension (gestational hypertension group, including 23 cases of gestational hypertension and 22 cases of mild preeclampsia) were detected by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry. , Severe preeclampsia in 23 cases) and 20 normal pregnant women (control group) serum and placental tissue Endoglin levels. Results Serum soluble Endoglin level in gestational hypertension group (29.66 ± 8.52 ng / ml) was significantly higher than that in control group (10.42 ± 4.35 ng / ml) (P <0.01). The levels of soluble Endoglin in gestational hypertension, mild preeclampsia and severe preeclampsia increased gradually (13.24 ± 6.17ng / ml, 28.28 ± 10.19ng / ml, 42.50 ± 13.48ng / ml respectively) There was significant difference between groups (all P <0.01). Compared with control group, the expression of Endoglin in placental tissue of hypertensive disorder complicating pregnancy was significantly increased (P <0.01). The levels of Endoglin in gestational hypertension, mild and severe preeclampsia placenta increased gradually, with significant difference between the groups (P <0.05). Serum Soluble Endoglin levels were positively correlated with placental Endoglin expression (r = 0.504, P <0.05 and r = 0.532, P <0.05). Conclusions Endoglin levels may be related to the pathogenesis and development of hypertensive disorder complicating pregnancy.