酶免疫组织化学技术,是以辣根过氧化物酶(Horsenadish peroxidase 简称HRP)结合在抗体、抗原或中间体上,对组织或细胞进行的免疫化学反应。由于酶对底物的对数放大作用和成色反应,增强了分辨力,使抗原抗体反应具有较高的的敏感性和特异性。本法具有操作简便,试剂稳定和不需用特殊设备等优点;在细胞涂片、冰冻切片和石腊切片中,均可在光镜或电镜下定位或定量组织细胞中的各种成份和研究细微结构等。因此,自1966年 Nakane 等建立酶免疫技术以来,发展很快。最初主要是通过化学交联剂将酶与抗体交联,用以检测组织中的相应抗原,相 Enzyme immunohistochemistry is an immunochemical reaction of tissues or cells by binding of horseradish peroxidase (HRP) to antibodies, antigens or intermediates. As the enzyme on the substrate of the logarithmic amplification and color reaction, enhanced resolution, the antigen-antibody reaction has a high sensitivity and specificity. The method has the advantages of simple operation, stable reagents and no special equipment. In cell smears, frozen sections and paraffin sections, various components in tissue cells can be located or quantified under light or electron microscope Subtle structure. Therefore, Nakane and other enzyme immunoassay since 1966, has developed rapidly. Initially mainly through the chemical cross-linking agent and antibody cross-linked to detect the organization of the corresponding antigen, phase