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A method was developed for the determination of tetraethyl ammonium(TEA) by reversed-phase ionpair chromatography with indirect ultraviolet detection.Chromatographic separation was achieved on a reversed-phase C18 column using background ultraviolet absorbing reagent-ion-pair reagent-organic solvent as mobile phase.The effects of the background ultraviolet absorbing reagents,detection wavelength,ion-pair reagents,organic solvents and column temperature on the determination method were investigated and the retention rules discussed.Results found that TEA could be successfully analyzed by using 0.7 mmol/L 4-aminophenol hydrochloride and 0.15 mmol/L 1-heptanesulfonic acid sodium mixed with 20%(v/v) methanol as mobile phase at a UV detection wavelength of 230 nm.Under these conditions,the retention time of tetraethyl ammonium was 2.85 min.The detection limit(S/N=3)for TEA was 0.06 mg/L.The relative standard deviations(n=5) for peak area and retention time were0.35%and 0.02%,respectively.The method has been successfully applied to the determination of synthesized tetraethyl ammonium bromide.Recovery of tetraethyl ammonium after spiking was 99.1%.
A method was developed for the determination of tetraethyl ammonium (TEA) by reversed-phase ionpair chromatography with indirect ultraviolet detection. Chromatographic separation was achieved on a reversed ultraviolet C18 column using ultraviolet absorbing reagent-ion-pair reagent-organic solvent as mobile phase. These effects of the background ultraviolet absorption reagents, detection wavelength, ion-pair reagents, organic solvents and column temperature on the determination method were investigated and the retention rules discussed. Results found that TEA could be successfully analyzed by using 0.7 mmol / L 4-aminophenol hydrochloride and 0.15 mmol / L 1-heptanesulfonic acid sodium mixed with 20% (v / v) methanol as mobile phase at a UV detection wavelength of 230 nm. Unit conditions, the retention time of tetraethyl ammonium was 2.85 min. The detection limit (S / N = 3) for TEA was 0.06 mg / L. The relative standard deviations (n = 5) for peak area and retention time were 0.35% and 0.02% respectively.Th e method has been successfully applied to the determination of synthesized tetraethyl ammonium bromide. Recovery of tetraethyl ammonium after spiking was 99.1%.