目的对了哥王炮制前生品及两种方法炮制后的炮制品的抗菌及抗炎活性进行比较,探讨两种炮制方法对了哥王药效的影响。方法分别以“汗渍法”、人工汗液炮制法炮制了哥王,制备了哥王生品及两种炮制品浸膏;采用体内及体外抗菌实验观察三种样品的抗菌活性;采用二甲苯致小鼠耳肿胀实验观察其抗炎活性。结果体外抗菌实验结果显示了哥王生品及两种炮制品对金黄色葡萄球菌及乙型溶血性链球菌均有抗菌作用;对金黄色葡萄球菌的MIC值是两种炮制品较小,MBC值是生品较小;对乙型溶血性链球菌的MIC与MBC值均是人工汗液炮制品最小。体内抗菌实验结果表明:三种样品均能降低金黄色葡萄球菌感染小鼠的死亡率,且三种样品的作用强弱无明显差异。抗炎实验结果表明三种受试样品均能抑制二甲苯所致的小鼠耳肿胀,各样品抑制作用强度无明显差异。结论了哥王生品及两种炮制品均具有抗菌及抗炎活性,且三种样品的作用强弱无明显差异,表明通过“汗渍法”及人工汗液炮制法对了哥王进行炮制后,未降低其抗菌及抗炎活性。 Objective To compare the antibacterial and anti-inflammatory activities of the processed products of the former king and the two processed basil, and to discuss the effects of two processing methods on the efficacy of the king. Methods The method of “perspiration stain” and artificial sweat preparation were used to make the king, the brother Wang products and two processed products extract were prepared. The antibacterial activities of the three samples were observed by in vitro and in vivo antibacterial experiments. Ear swelling test observed its anti-inflammatory activity. Results The results of in vitro antibacterial test showed that the crude product of King and the two processed products had antibacterial activity against Staphylococcus aureus and Hemolytic streptococcus; the MIC value of Staphylococcus aureus was smaller for both processed products and the MBC value was Health products smaller; MIC for the hemolytic streptococcus B and MBC values ​​are artificial sweetener products minimum. In vivo antibacterial experiment results show that: all three samples can reduce the mortality of mice infected with Staphylococcus aureus, and the strength of the three samples showed no significant difference. Anti-inflammatory experimental results show that the three tested samples can inhibit xylene-induced mouse ear swelling, the inhibitory effect of each sample was no significant difference. It is concluded that the bigeye product and the two processed products have antibacterial and anti-inflammatory activity, and there is no significant difference in the strength of the three samples, which indicates that after the processing of the biotech by “Perspiration Method” and artificial sweat processing method, Did not reduce its antimicrobial and anti-inflammatory activity.