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目的探讨凉血活血方对卵巢癌SKOV3细胞的凋亡作用及其机制。方法将凉血活血方药液(浓度为1.125,2.228,3.31,4.37,5.41 mg·m L~(-1))作用于体外培养的SKOV3细胞,采用MTT法观察24 h及48 h细胞增殖的情况。流式细胞术观察凉血活血方对SKOV3细胞凋亡的影响;应用Western bolt法检测细胞中凋亡蛋白caspase-3的表达情况。结果 1MTT结果表明不同浓度药物作用于细胞24 h,48 h后实验组对细胞的抑制率均高于空白对照组(P<0.05);2凋亡结果显示2.228,4.37,5.41 mg·m L-1实验组凋亡率均高于空白对照组(P<0.05);3Western bolt显示各实验组蛋白样品中caspase-3的表达高于空白对照组(P<0.05)。结论一定浓度的凉血活血方能够抑制SKOV3细胞的增殖,促进其凋亡,其机制是通过上调caspase-3的表达实现的。
Objective To investigate the apoptosis of ovarian cancer SKOV3 cells and its mechanism. Methods Liang Xue Huo Xue Fang liquid (concentration of 1.125,2.228,3.31,4.37,5.41 mg · m L -1) in vitro cultured SKOV3 cells MTT method was used to observe the cell proliferation at 24 h and 48 h Happening. Flow cytometry was used to observe the effect of cooling blood and activating blood recipe on apoptosis of SKOV3 cells. Western blot was used to detect the expression of caspase-3. Results The results of 1 MTT showed that the inhibitory rates of cells treated with different concentrations of drugs for 24 h and 48 h were higher than those of blank control group (P <0.05); 2 The results of apoptosis showed that the inhibitory rates of 2.228, 4.37 and 4.41 mg · L- 1 in experimental group was higher than that in blank control group (P <0.05). The expression of caspase-3 in 3 groups was higher than that in blank control group (P <0.05) by 3Western bolt. Conclusion A certain concentration of Xuexue Huoxue Prescription can inhibit SKOV3 cell proliferation and promote its apoptosis by up-regulating the expression of caspase-3.