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目的 研究人源性细胞在电磁脉冲 (EMP)及电离辐射作用后的生物效应 .方法 用电磁脉冲发生器和6 0 Co-γ线分别照射人肠上皮细胞 ,以四唑盐 (MTT)比色法检测受照细胞的存活程度 .结果 电磁脉冲 (EMP)作用后 ,EMP电场场强为 90 k V· m- 1 时 ,随着脉冲次数增加 (1～ 15次 )条件下 ,细胞存活率逐渐降低 ,以 15次组最为明显 (P<0 .0 1) ,该组细胞于照后 96 h,其存活率仅为对照组的 80 .0 % (P<0 .0 1) ;电离辐射作用后 (4～ 12 Gy范围内 ) ,随照射剂量增加 ,细胞存活率 2 4h时各组与对照组相差不显著 ,但 96 h时各组均低于对照组 (P<0 .0 1) .结论 本实验条件下 ,电磁脉冲和电离辐射均可降低细胞的存活率 ,随照后时间的延长 ,前者呈回升趋势 ,后者则呈进行性降低 .
Objective To study the biological effects of human-derived cells (EMPs) and ionizing radiation (IMRT) on human intestinal epithelial cells.Methods The human intestinal epithelial cells were irradiated with electromagnetic pulse generator Method was used to test the survival of irradiated cells.Results When the field intensity of EMP was 90 kV · m-1 after EMP, the cell survival rate gradually increased with the increase of pulse times (1 ~ 15 times) (P <0.01). The survival rate of this group was only 80.0% (P <0.01) of the control group at 96 h after irradiation. The effect of ionizing radiation (Within the range of 4 ~ 12 Gy). With the increase of radiation dose, the cell survival rate was no significant difference between the two groups at 24 hours, but it was lower than the control group at 96 hours (P <0.01). Conclusion Under the experimental conditions, both the electromagnetic pulse and ionizing radiation can reduce the cell survival rate, with the extension of time after the irradiation, the former showed a upward trend, while the latter showed a progressive decline.