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目的:研究口腔鳞状细胞癌中p75神经营养因子受体(p75NTR)的表达,探讨p75NTR作为预测口腔鳞状细胞癌预后指标及将其作为肿瘤干细胞标志物的可能性。方法:采用SP免疫化学方法,对舌鳞状细胞癌细胞系Tca8113及43例手术切除口腔鳞状细胞癌标本进行p75NTR表达的检测,另外选取5例癌旁组织及8例正常组织作为对照。应用SPSS16.0软件包,采用行×列表资料的χ2检验进行统计学分析。结果:p75NTR在Tca8113细胞膜中阳性表达。43例口腔鳞状细胞癌组织中,p75NTR阳性表达33例,在癌旁组织及正常对照组中没有表达。p75NTR均定位于细胞膜上,于癌巢中分散分布,且常有聚集性分布趋势。p75NTR表达与临床分期(P<0.05)及有无淋巴结转移(P<0.05)相关。结论:p75NTR的表达可以作为预测口腔鳞状细胞癌预后的指标,但还不能将其单独作为肿瘤干细胞的标志物,其在肿瘤发生、发展中的作用及机制仍需进一步研究。
OBJECTIVE: To study the expression of p75 neurotrophic factor receptor (p75NTR) in oral squamous cell carcinoma and to explore the possibility of p75NTR as a prognostic indicator of oral squamous cell carcinoma (OSCC) and to use it as a tumor stem cell marker. Methods: Immunohistochemical SP method was used to detect the expression of p75NTR in tongue squamous cell carcinoma cell line Tca8113 and 43 cases of surgical resection of oral squamous cell carcinoma. In addition, 5 cases of paracancerous tissues and 8 cases of normal tissues were selected as control. Application SPSS16.0 software package, the use of × × × × test data table for statistical analysis. Results: p75NTR was positively expressed in Tca8113 cell membrane. In 43 cases of oral squamous cell carcinoma, p75NTR positive expression in 33 cases, adjacent tissues and normal control group was not expressed. P75NTR are located in the cell membrane, scattered in the cancer nest distribution, and often aggregated distribution trend. The expression of p75NTR was correlated with clinical stage (P <0.05) and lymph node metastasis (P <0.05). CONCLUSION: The expression of p75NTR can be used as an index to predict the prognosis of oral squamous cell carcinoma, but it can not be used as a marker of tumor stem cells alone. The role and mechanism of p75NTR in tumorigenesis still need to be further studied.