论文部分内容阅读
以番茄环斑病毒阳性样品为研究材料,根据GeneBank(核酸序列数据库)中的序列设计特异性引物进行IC-RTP-CR(免疫反转录聚合酶链式反应,下同)扩增,获得产物克隆到pMD18-T载体中,经测序后与目标序列(ToRSVL19655)比较,核苷酸同源性为87.3%。通过实验建立了检测该病毒的IC-RT-PCR方法。该方法对其他样品核酸提取困难而抗体较容易制备的病毒检测同样具有借鉴意义。
The positive sample of tomato ring spot virus was used as the research material, and specific primers were designed according to the sequences in GeneBank to amplify IC-RTP-CR (the same below), to obtain the product Was cloned into pMD18-T vector and sequenced to compare with the target sequence (ToRSVL19655), the nucleotide homology was 87.3%. The IC-RT-PCR method for detecting the virus was established through experiments. This method is also of reference for the detection of viruses that are difficult to extract from other samples and antibodies are easier to prepare.