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目的进行第七批细菌内毒素国家标准品原料制备方法的研究。方法采用4种方法制备内毒素,对得到的内毒素原料进行理化分析后,选取其中最好的制备方法,再采用正交法对其实验条件进行优化。结果最终方法确定为:复苏、培养和收获大肠杆菌;热酚水法粗提;超速离心(4℃,105000×g,4h)纯化;冻干得到原料。结论确立了第七批细菌内毒素国家标准品原料的制备方法。
Objective To study the preparation method of the seventh batch of bacterial endotoxin national standard raw materials. Methods Four kinds of methods were used to prepare endotoxin. After the physical and chemical analysis of the obtained endotoxin raw materials, the best preparation method was selected and then the orthogonal test was used to optimize the experimental conditions. Results The final results were as follows: recovery, culture and harvest of E. coli; hot phenol extraction by crude water; purification by ultracentrifugation (4 ℃, 105000 × g, 4h); Conclusion The preparation of the seventh batch of bacterial endotoxin national standard raw materials was established.