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目的 研究增殖细胞核抗原 ( PCNA)在口腔粘膜上皮癌变过程中的表达及其在癌变诊断中的意义。方法 采用免疫组化 S-P法对正常口腔粘膜、轻中重度粘膜上皮异常增生和高分化鳞癌共 85例标本进行 PCNA检测。结果 正常口腔粘膜基底细胞层可有少量 PCNA阳性细胞 ;而上皮异常增生和鳞癌时 ,随着病变程度加重 ,PCNA阳性表达也相应提高 ,且阳性反应出现在基层以上部位 ;尤其是重度异常增生 ,其 PCNA阳性表达率较中度异常增生明显增强 ( P=0 .0 0 0 8) ,而重度异常增生与鳞癌间 ,PCNA表达无显著性差异 ( P=0 .72 15 )。结论 PCNA表达在口腔粘膜上皮癌变诊断中有重要参考价值 ,可作为监测其恶性变的重要生物学指标
Objective To study the expression of proliferating cell nuclear antigen (PCNA) in carcinogenesis of oral mucosa and its significance in the diagnosis of canceration. Methods Immunohistochemical S-P method for normal oral mucosa, mild to moderate mucosal epithelial dysplasia and well-differentiated squamous cell carcinoma in 85 cases were detected by PCNA. Results The normal oral mucosa basal cell layer may have a small number of PCNA positive cells; while epithelial dysplasia and squamous cell carcinoma, as the degree of lesion increased, the positive expression of PCNA also increased correspondingly, and the positive reaction appeared above the basal layer, especially severe dysplasia . The positive rate of PCNA expression was significantly higher than that of moderate degree abnormality (P = 0.0008), but there was no significant difference between severe dysplasia and squamous cell carcinoma (P = 0.7215). Conclusion The expression of PCNA has important reference value in the diagnosis of oral mucosal carcinogenesis and can be used as an important biological indicator to monitor the malignant transformation