从蛇胆中分得胆酸和牛磺胆酸,并做了化学性质和物理常数测试。实验采用胆盐和胆汁酸的薄层层析(TLC)、胆盐的柱层析、胆盐的碱水解、胆汁酸的反相分配柱层析等技术。胆酸的化学结构通过IR、MS、~1H-NMR 等物理测试证实。胆盐碱水解水相产物纸层析和 TLC表明与胆酸及其它胆汁酸结合的氨基酸为牛磺酸,蛇胆汁中胆酸的天然存在形式为牛磺胆酸。本文从分离和结构鉴定角度证实蛇胆汁的主要成分为牛磺胆酸。 Bile acids and taurocholic acid were separated from snake gall bladders and tested for chemical properties and physical constants. The experiments used thin layer chromatography (TLC) of bile salts and bile acids, column chromatography of bile salts, alkali hydrolysis of bile salts, and reverse column chromatography of bile acids. The chemical structure of cholic acid was confirmed by physical tests such as IR, MS and ~1H-NMR. Paper chromatography and TLC of the aqueous product of bile salt alkaline hydrolysis showed that the amino acid bound to cholic acid and other bile acids was taurine, and the naturally occurring form of cholic acid in snake bile was taurocholic acid. From the perspective of separation and structure identification, the main component of snake bile is taurocholic acid.