论文部分内容阅读
目的 检测 46例恶性血液病患者化疗前后外周血T细胞亚群及NK细胞活性 ,观察胸腺肽免疫功能。方法 应用单克隆抗体和流式细胞仪。结果 (1)恶性血液病患者各项免疫活性细胞表达均低于对照组 ;(2 )晚期和复发型恶性血液病患者各项免疫指标均低于初发型恶性血液病 ;(3 )AL各项免疫指标明显低于其它恶性血液病 ;(4 )治疗后A、B两组比较 ;A组的NK细胞活性明显高于B组 ,A组治疗后CD3 、CD4、CD4/CD8、NK细胞活性明显高于治疗前。结论 T细胞亚群、NK细胞活性在恶性血液病患者中受到抑制并随病情程度而进一步加剧 ,其中AL患者受抑更甚 ,胸腺肽能明显提高恶性血液病患者的细胞免疫功能 ,尤其对NK细胞 ,它是一种低毒高效的生物反应调节剂。
Objective To detect the activity of T lymphocyte subsets and NK cells in peripheral blood of 46 patients with hematologic malignancies before and after chemotherapy and observe the immunological function of thymosin. Methods Monoclonal antibodies and flow cytometry were used. Results (1) The expression of various immunocompetent cells in patients with hematologic malignancies was lower than that of the control group. (2) The immunological indexes in patients with advanced and recurrent hematologic malignancies were lower than those in patients with primary hematologic malignancies. (3) Immune index was significantly lower than other hematologic malignancies; (4) after treatment A, B two groups compared; A group of NK cell activity was significantly higher than the B group, A group after treatment CD3, CD4, CD4 / CD8, NK cell activity was significantly Higher than before treatment. Conclusion T cell subsets and NK cell activity are inhibited in patients with hematologic malignancies and further aggravated with the severity of the disease. Among them, patients with AL are more depressed, thymosin can significantly improve cellular immune function in patients with hematologic malignancies, especially NK cells , It is a low toxicity and efficient biological response modifier.