Objective:To predict the protein similarity, biological function and structure of 116 kDa protein that was isolated from human sperm membrane of acrosome by usingin silico. Methods:Predictions for 116 kDa protein similarity was done through comparative analysis of its isoelectric point (pI) and molecular weight to the UniProtKB/TrEMBL protein database. The three-dimensional structure of the protein was built using SWISS-MODEL.Results:The 116 kDa protein with pI 4.4 was expected to have similarities with CDH23 proteins that had pI 4.38, thus they likely had similar physical and biochemical properties. The predicted protein had an extracellular N-terminal that likely acted as a receptor, and showed cell recognition characteristic, playing a role in fertilization.Conclusions:The 116 kDa protein isolated from acrosome membrane has similarity on its biophysical character to the CDH23.