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作者采用小鼠伯氏疟原虫为模型,对脑型疟引起的大脑损伤局部分布和类型的关系进行系统研究。用10~5个感染伯氏疟原虫K_(178)株的红细胞腹腔接种90只近交雌性小鼠。于每天上午和下午每隔12小时观察一次感染的临床过程。病重小鼠以过量乙醚致死,迅速取出鼠脑,再横向切成4片,浸在卡诺氏溶液中固定。将石腊包埋的标本切成5μm,按Goldner方法用甲基绿派若宁、P.A.S.、苏木精和伊红染色。大脑出现明显改变的有48只小鼠,光镜检查损伤处,并作局部定位。作者发现除严重脑水肿外,主要的形态变化有微血栓形成、单核细胞沉积、小动脉痉
In this study, we used a mouse model of Plasmodium berghei to systematically study the relationship between the local distribution and types of brain injury caused by cerebral malaria. Ninety inbred female mice were inoculated intraperitoneally with 10 to 5 erythrocytes infected with P. berghei K_ (178) strain. The clinical course of the infection was observed every 12 hours every morning and afternoon. Severe mice died of excess ether, the rat brain was quickly removed, and then cut into four transverse, immersed in Carnot’s solution in the fixed. The paraffin-embedded specimens were cut to 5 μm and stained with methylprednisolone, P.A.S., hematoxylin and eosin by the Goldner method. There were 48 mice with significant changes in the brain. The lesions were examined by light microscopy and localized. The authors found that in addition to severe brain edema, the main morphological changes were micro-thrombosis, mononuclear cell deposition, arteriolar spasm