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Objective:The aim of our study was to investigate the relationship between cell apoptosis and dephosphorylated RB protein and proliferating cell nuclear antigen(PCNA) in human breast cancer.Methods:MTT colorimetric assay was applied to examine the growth inhibition,and the apoptosis was determined by flow cytometry(FCM).The expressing quantity of dephosphorylated RB protein and PCNA pre-and post the action of ADR were detected with immunocytochemistry.Results:MTT assay revealed that ADR inhibited proliferation of MCF-7/S cells in a dose dependent manner,the 50% inhibition concentration(IC50) value was 0.128 mg/L.Tumor cell apoptotic rate(AR) in ADR group(χ= 0.259) was significantly higher than that in the control group(χ = 0.045)(P < 0.01).The expressive levels of dephosphorylated RB protein in ADR group(MOD × area = 986.8 ± 207.4) was significantly higher than that in control group(MOD × area =131.7 ± 31.9)(P < 0.01).PCNA positive expression rate in ADR group(χ = 0.3371) was significantly lower than that in the control group(χ = 0.5152)(P < 0.01).Conclusion:In ADR group,there was significant positive correlation between AR and the expressing quantity of dephosphorylated RB protein,but there was significant negative correlation between AR and PCNA.
Objective: The aim of our study was to investigate the relationship between cell apoptosis and dephosphorylated RB protein and proliferating cell nuclear antigen (PCNA) in human breast cancer. Methods: MTT colorimetric assay was applied to examine the growth inhibition, and the apoptosis was determined by flow cytometry (FCM). The amount of dephosphorylated RB protein and PCNA pre-and post the action of ADR were detected with immunocytochemistry. Results: MTT assay revealed that ADR inhibited proliferation of MCF-7 / S cells in a stressed manner , the 50% inhibition concentration (IC50) value was 0.128 mg / L. Tumor cell apoptotic rate (AR) in ADR group (χ = 0.259) was significantly higher than that in the control group The expressive levels of dephosphorylated RB protein in ADR group (MOD × area = 986.8 ± 207.4) were significantly higher than those in control group (MOD × area = 131.7 ± 31.9) (P <0.01) (χ = 0.3371) was si gnificantly lower than that in the control group (χ = 0.5152) (P <0.01) .Conclusion: In ADR group, there was a significant positive correlation between AR and the expressing quantity of dephosphorylated RB protein, but there was a significant negative correlation between AR and PCNA.