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目的:将不锈钢微针阵列应用于经皮给药。考察离体大鼠皮肤经不同针形微针预处理相同时间、相同针形微针预处理不同时间后,模型药物鬼臼毒素经大鼠皮肤的透皮能力。方法:微针预处理大鼠皮肤后,用改进的Franz扩散池研究鬼臼毒素对皮肤的透皮速率。高效液相色谱法测定鬼臼毒素的含量。结果:皮肤经微针预处理后进行鬼臼毒素透皮,其透皮速率比未经微针处理时有明显提高。三角形微针、梯形微针、矛形微针对鬼臼毒素的促渗能力依次增强;三者所引起的鬼臼毒素在皮肤中的滞留量有显著差异。同种针形微针预处理皮肤时间越长,鬼臼毒素的透皮速率越大;但微针预处理时间对皮肤中的药物滞留量无显著影响。结论:微针用于药物经皮给药时,微针针形、微针的预处理时间对药物的经皮渗透具有重要影响。
Objective: To apply stainless steel microneedle array to transdermal delivery. Examine the ex vivo rat skin by different acupuncture microneedles pretreatment at the same time, the same needle microneedle pretreatment at different times, the model drug podophyllotoxin through the skin of rats transdermal ability. Methods: After the microneedles were pretreated on the skin of rats, a modified Franz diffusion cell was used to study the transdermal rate of podophyllotoxin to the skin. Determination of Podophyllotoxin by High Performance Liquid Chromatography. Results: The skin was transplanted with podophyllotoxin after microneedle pretreatment. The transdermal rate of the skin was significantly higher than that without microneedle treatment. Triangular microneedles, trapezoidal microneedles and spear microneedles enhanced the potent propensity of podophyllotoxins in turn. There were significant differences in the retention of podophyllotoxin in the skin caused by the three. The same acicular microneedles pretreatment of the skin longer, the greater the rate of podophyllotoxin transdermal; but microneedle pretreatment time on the skin retention of drugs had no significant effect. Conclusion: When microneedles are used in transdermal drug delivery, the pretreatment time of microneedles and microneedles has an important influence on transdermal drug penetration.