目的　检测分析培养的胚胎早期视网膜神经细胞游离钙 (Ca2 + )分布与钙 (Ca2 + )通道特征。　方法　体外培养 11～ 15周胎儿视网膜神经细胞 ,在含 Ca2 +与不含 Ca2 +的 Hepes缓冲液中与钙荧光指示剂 Fluo3孵育染色 ,同时加入或不加入异博定、佩尔地平或地塞米松 ,共聚焦显微镜观察记录游离 Ca2 +分布 ,以及受不同浓度 K+刺激后 Ca2 +通道开放与 Ca2 +转移情况。　结果　培养的 11～ 15周胎儿视网膜神经元及神经胶质细胞受 K+ 刺激后均出现明显的 Ca2 + 转移与再分布 ,在缺乏细胞外 Ca2 + 的情况下 ,细胞浆内钙库仍可迅速释放 Ca2 +并转移至细胞核内。异博定、佩尔地平及地塞米松能够抑制细胞外 Ca2 +进入视网膜神经细胞内。　结论　胚胎早期视网膜神经元及神经胶质细胞存在 L型 Ca2 + 通道 ,并已基本发育成熟 Objective To detect and analyze the distribution of calcium (Ca2 +) and calcium (Ca2 +) channels in early embryonic retinal neurons. Methods Fetal retinal neurons cultured in vitro for 11 ~ 15 weeks were stained with calcium fluorescence indicator Fluo3 in Ca2 + -containing and Ca2 + -containing Hepes buffer with or without isoboridine, perdipine or dexamethasone Miscell and confocal microscopy were used to observe the distribution of free Ca2 + and the Ca2 + channel opening and Ca2 + transfer after stimulation with different concentrations of K +. Results Fetal retinal neurons and glial cells cultured in 11 ~ 15 weeks showed significant Ca2 + transfer and redistribution after stimulated by K +. In the absence of extracellular Ca2 +, cytoplasmic calcium stores were still rapidly released Ca2 + and transferred to the nucleus. Diflubenzuron, Perdipine and dexamethasone can inhibit the extracellular Ca2 + into the retinal nerve cells. Conclusion There are L-type Ca2 + channels in early embryonic retinal neurons and glial cells and they have basically matured