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观察细辛木脂素联合供体脾细胞对同种异体心脏移植免疫耐受的诱导效果,为抗排斥药物的研究提供依据。方法:分别采用供体脾细胞、细辛木脂素(ＨＡＬ)和ＨＡＬ联合供者脾细胞预处理移植受体,然后行大鼠异位心脏移植术。根据实验各组移植心脏存活时间和混合淋巴细胞反应进行观察。结果:对照组、脾细胞组、ＨＡＬ组和ＨＡＬ＋脾细胞组的移植心脏存活天数分别为(６．８１±０．９７)ｄ,(７．２０±１．５５)ｄ,(１０．４０±２．７８)ｄ,(２７．６３±６．４６)ｄ,ＨＡＬ＋脾细胞组的移植心脏存活时间明显长于脾细胞组和 ＨＡＬ组(Ｐ＜０．０１);移植术后７ｄ和１４ｄ的混合淋巴细胞反应以ＨＡＬ＋脾细胞组的ＣＰＭ值为最低(Ｐ＜０．０１)。结论:细辛木脂素能够明显提高供体脾细胞所诱导的免疫耐受强度,显著地延长大鼠同种异移植体心脏的存活时间。
To observe the inducing effect of Asiolignan combined with donor spleen cells on immune tolerance of allogeneic heart transplantation and provide the basis for the study of anti-rejection drugs. Methods: The donor spleen cells, asarosin (HAL) and HAL combined donor spleen cells were used to pretreat recipients and then subjected to heterotopic heart transplantation in rats. According to the experimental group, allograft survival time and mixed lymphocyte reaction were observed. Results: The survival days in control group, spleen cell group, HAL group and HAL + splenocyte group were (6.81 ± 0.97) d, (7.20 ± 1.55) d, (10.40 ± 2.78) d, (27.63 ± 6.46) d, the survival time of HAL + splenocyte transplantation group was significantly longer than that of splenocyte group and HAL group (P <0.01). After 7 and 14 days of transplantation, The CPM value of lymphocyte reaction in HAL + splenocyte group was the lowest (P <0.01). CONCLUSION: Asarixin can significantly improve the immune tolerance induced by donor spleen cells and prolong the survival time of allograft heart in rats significantly.