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为了研究接触室内煤烟污染的肺癌患者的发病机制，我们采用酶连免疫斑点法检测了接触室内煤烟的１９例肺癌患者（Ａ组）、２２例未患肺癌者（Ｂ组）和１９例不接触煤烟的未患肺癌者（Ｃ组）血清中７种细胞生长调控基因编码的蛋白。结果表明，Ａ组ｒａｓ、ｐ５３和ｎｅｕ蛋白含量明显高于Ｂ、Ｃ组，差异有显著性（Ｐ＜０．０５）或极显著性（Ｐ＜０．０１）；Ａ组的阳性样品数为ｒａｓ蛋白６例（其中２例为强阳性，ｐ５３、ｎｅｕ和ｊｕｎ蛋白各３例，ｐ１６蛋白１例；Ｂ组的阳性样品数为ｒａｓ蛋白２例，ｐ１６蛋白１例。提示血清中细胞生长调控蛋白可作为环境相关肺癌的生物标志。
In order to study the pathogenesis of lung cancer patients exposed to indoor smoke pollution, we used enzyme-linked immunospot assay to detect 19 patients with lung cancer (Group A), 22 patients without lung cancer (Group B) and 19 patients exposed to indoor soot. The proteins encoded by the seven cell growth regulatory genes in the sera of patients without lung cancer (C) without exposure to soot. The results showed that the protein content of ras, p53 and neu in group A was significantly higher than that in group B and C. The difference was significant (P<0.05) or extremely significant (P<0.01). The number of positive samples in group A was There were 6 cases of ras protein (2 cases were strongly positive, 3 cases were p53, 3 cases of neu and jun proteins, 1 case of p16 protein; 2 cases of ras protein and 1 case of p16 protein were positive in group B. It suggested that the regulation of cell growth in serum. Protein can serve as a biomarker for environmental-related lung cancer.