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[目的]探讨莪术对大鼠胃动力作用机制及脑肠肽的调节作用。[方法]40只大鼠随机分成正常对照组、模型组、25%莪术组、50%莪术组和0.1%多潘立酮组,每组8只。正常对照组以外的各组采用不规则进食加稀盐酸饲养制作胃电节律失常模型,并给予相应处理,4周后观察大鼠胃肌电活动异常节律指数和慢波频率变异系数、胃排空率,放免法测定胃窦及空肠组织胃动素(MOT)、血管活性肠肽(VIP)的水平。[结果]与正常对照组比较,模型组肌电活动异常节律指数和慢波频率变异系数明显升高(P<0.01),胃排空率下降(P<0.01);MOT在胃窦、空肠组织的水平下降,而VIP水平则上升(P<0.01)。经25%莪术水煎剂治疗后上述各值均恢复或接近正常,与多潘立酮组比较差异无统计学意义(P>0.05),与模型组比较差异有统计学意义(P<0.01)。[结论]25%莪术水煎剂有明显的促胃动力作用,其机制可能与其调节肽能神经的体液,改善胃电节律有关。
[Objective] To investigate the mechanism of gastric motility and the regulation effect of brain-gut peptide in rats with Curcuma zedoaria. [Methods] Forty rats were randomly divided into normal control group, model group, 25% curcumin group, 50% curcumin group and 0.1% domperidone group, with 8 rats in each group. Rats outside the normal control group were fed with irregular diets and dilute hydrochloric acid to produce a gastric dysrhythmia model and treated accordingly. After 4 weeks, the abnormal rhythm index and frequency coefficient of slow-wave frequency, gastric emptying were observed in rats. The radioimmunoassay was used to determine the levels of motilin (MOT) and vasoactive intestinal peptide (VIP) in antrum and jejunum. [Results] Compared with the normal control group, the abnormal rhythm index and slow wave frequency variation coefficient of myoelectric activity in the model group were significantly higher (P<0.01), and the gastric emptying rate was decreased (P<0.01); MOT was in gastric antrum and jejunum tissue The level of decline decreased while the level of VIP increased (P<0.01). After treatment with 25% Rhizoma Curcumae, the above values were all recovered or nearly normal, and there was no statistically significant difference from the domperidone group (P>0.05), and there was a statistically significant difference from the model group (P<0.01). [Conclusion] 25% Rhizoma Curcumae decoction has obvious gastric motility and its mechanism may be related to its regulation of body fluid of peptide nerves and improvement of gastric rhythm.