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目的检测Ｐ糖蛋白在不同胆囊组织中的表达，以探讨该蛋白与胆囊癌抗化疗特性的关系及作为胆囊癌生物标志物的潜在价值。方法采用碱性磷酸酶抗碱性磷酸酶（ＡＰＡＡＰ）的免疫组化方法，用二种决定簇不同的抗人Ｐ糖蛋白的单克隆抗体ＪＳＢ１和ＵＩＣ２，检测２６例胆囊癌、１４例胆囊良性肿瘤及９例正常胆囊组织中Ｐ糖蛋白的表达情况，并分析其表达程度与不同临床资料的相关关系。结果单抗ＪＳＢ１测得Ｐ糖蛋白在胆囊癌中表达的阳性率为７６．９％，显著高于胆囊良性肿瘤组及正常胆囊组（阳性率分别为３５．７％和３３．３％，Ｐ＜０．０５）。单抗ＵＩＣ２测得Ｐ糖蛋白在胆囊癌中表达的阳性率为６９．２％，也显著高于胆囊良性肿瘤组及正常胆囊组（阳性率分别为２１．４％和１１．１％，Ｐ＜０．０１）。Ｐ糖蛋白的表达阳性率与胆囊癌的ＴＮＭ分期及胆囊良恶性肿瘤的病理类型均无相关关系。结论Ｐ糖蛋白在胆囊恶性肿瘤中的过表达，提示其在胆囊癌对化疗不敏感的特性中起着重要作用，同时Ｐ糖蛋白在不同胆囊组织中表达的显著差异性，也提示其作为胆囊恶性肿瘤的生物标志物的价值
Objective To detect the expression of P glycoprotein in different gallbladder tissues and to explore the relationship between the protein and gallbladder cancer anti-chemotherapy characteristics and its potential value as a biomarker for gallbladder cancer. METHODS: The alkaline phosphatase-anti-alkaline phosphatase (APAAP) immunohistochemical method was used to detect 26 cases of gallbladder with two different determinants of monoclonal antibodies against human P glycoprotein JSB-1 and UIC-2. The expression of P glycoprotein in cancer, 14 cases of benign gallbladder tumors and 9 cases of normal gallbladder tissues were analyzed, and the correlation between the expression levels and clinical data was analyzed. Results The positive rate of P glycoprotein expression in gallbladder carcinoma was 76.9%, which was significantly higher than that of the gallbladder benign tumor group and normal gallbladder group (the positive rates were 35.7% and 33.3, respectively). %, P < 0.05). The positive rate of P glycoprotein expression in gallbladder carcinoma was 69.2% measured by monoclonal antibody UIC 2 and was also significantly higher than that of benign gallbladder tumor group and normal gallbladder group (positive rate was 21.4% and 11.1, respectively). %, P < 0.01). There was no correlation between the positive rate of P glycoprotein expression and the TNM staging of gallbladder carcinoma and the pathological types of benign and malignant gallbladder tumors. Conclusion Overexpression of P glycoprotein in gallbladder malignant tumors suggests that it plays an important role in the insensitivity of gallbladder carcinoma to chemotherapy. Meanwhile, the significant difference of P glycoprotein expression in different gallbladder tissues also suggests Value as a biomarker for gallbladder malignancy