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目的观察糖尿病大鼠肾脏中热休克蛋白70(HSP70)的表达并观察普罗布考对HSP70表达的影响。方法链脲佐菌素构建糖尿病大鼠模型50只,随机分为糖尿病(DM)组、普罗布考(PT)组,另设健康对照(NC)组。RT-PCR测定各组肾组织HSP70mRNA的表达,免疫组织化学法观察HSP70的表达情况,Western blot测定HSP70蛋白的表达水平。结果 DM组4、8及12周肾脏中HSP70mRNA及蛋白的表达逐渐升高。同一时间的PT组肾脏HSP70mRNA及蛋白的表达均较DM组减少(P<0.05)。结论糖尿病大鼠肾脏中HSP70的表达升高,普罗布考可降低HSP70的表达,对肾脏起保护作用。
Objective To observe the expression of heat shock protein 70 (HSP70) in kidney of diabetic rats and observe the effect of probucol on HSP70 expression. Methods Streptozotocin (STZ) was used to establish 50 diabetic rats. The rats were randomly divided into diabetic group (DM) and probucol (PT) group and another healthy control (NC) group. The expression of HSP70 mRNA in renal tissues was detected by RT-PCR. The expression of HSP70 was observed by immunohistochemistry. The expression of HSP70 protein was detected by Western blot. Results At 4, 8 and 12 weeks, the expression of HSP70 mRNA and protein in the kidney of DM group increased gradually. At the same time, the expression of HSP70mRNA and protein in the kidney of PT group were lower than those in DM group (P <0.05). Conclusion The expression of HSP70 in the kidneys of diabetic rats is increased. Probucol can reduce the expression of HSP70 and play a protective role on the kidneys.