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采用人精子与去透明带地鼠卵受精方法 ,分析诱变剂对人精子染色体与 2细胞胚微核的比较 ,以探讨染色体畸变率与微核率间关系。结果 :平阳霉素 (-S9mix) 1 0、2 0、40、60μg/ ml 4个剂量组染色体畸变率分别为 1 1 .5 %、2 5 .4%、3 3 .6%、43 .7% ;2细胞胚微核率分别为 42 .6%、49.1 %、5 9.6%、67.9%。环磷酰胺 (+ S9mix) 1 0、2 0、40、60μg/ml4个剂量组染色体畸变率分别为 1 1 .7%、1 8.5 %、3 1 .7%、42 .1 % .;2细胞胚微核率分别为 43 .40 %、5 4.0 %、65 .8%、69.3 %。与空白对照组比较 ,有显著差异 (P<0 .0 1 )。表明人精子染色体畸变率与 2细胞胚微核率之间存在相关性 ,均可检测诱变物对精子的损伤。
Human sperm and zona pellucida fertilization methods were used to compare the mutagenicity of human sperm chromosome with that of 2-cell embryos to explore the relationship between chromosome aberration rate and micronucleus rate. Results: The chromosomal aberrations in the four dose groups of Pingyangmycin (S9mix) 1 0, 2, 0, 40 and 60μg / ml were 11.5%, 25.4%, 33.6% and 43.7, respectively %. The micronuclei rates of 2-cell embryos were 42.6%, 49.1%, 59.6%, 67.9%, respectively. Cyclophosphamide (+ S9mix) 1 0,2 0,40,60μg / ml 4 doses of chromosome aberration rates were 11.7%, 8.5%, 31.7%, 42.1%; 2 cells The micronuclei rates of embryos were 43.4%, 5 4.0%, 65.8% and 69.3% respectively. Compared with the blank control group, there was a significant difference (P <0.01). The results showed that there was a correlation between the rate of chromosome aberration in human sperm and the rate of micronuclei in 2-cell embryos, which could detect the damage of mutagen to sperm.