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目的对支气管哮喘患儿血清中IFN-γ、IL-13及总IgE在哮喘发病机制中的相互关系及作用进行探讨。方法选择42例支气管哮喘儿童为观察组,42例健康儿童为对照组,对血清IFN-γ、IL-13及总lgE水平进行检测,采用双抗体夹心酶联免疫吸附法(ELISA)。支气管哮喘患儿缓解期、急性期做肺要进行功能检查。结果①支气管哮喘组患儿FEV1与用力肺活量(FVC)的比值(%)、血清IFN-γ与同期肺功能第1秒用力容积(FEV1)及最大呼气流量(PEF),均呈负相关关系(P值均<0.01)。②急性期发作期与缓解期的支气管哮喘组患儿肺功能的各项指标比较,(P值均<0.01)差异均有统计学意义。③支气管哮喘组患儿的急性发作期与缓解期比较,急性发作期、缓解期与对照组比较,总IgE水平、IFN-γ、血清IL-13差异具有统计学意义(P值均<0.01)。④支气管哮喘组患儿急性发作期、缓解期IL-13、总lgE与IFN-γ呈负相关关系。④支气管哮喘组患儿血清IL-13及总lgE水平与同期肺功能第1秒用力容积(FEV1)、FEV1与用力肺活量(FVC)的比值(%)及最大呼气流量(PEF)均呈正相关关系(P值均<0.01);结论血清中IL-13、IFN-γ、总IgE变化水平与哮喘病程存在相关性,共同参与病理生理过程,可作为病情严重程度客观指标。
Objective To investigate the relationship between the serum levels of IFN-γ, IL-13 and total IgE in the pathogenesis of asthma in children with bronchial asthma. Methods 42 children with bronchial asthma were selected as the observation group and 42 healthy children as the control group. Serum levels of IFN-γ, IL-13 and total IgE were detected by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). Bronchial asthma in children with remission, acute lung function to do a check. Results ① The ratio of FEV1 to FVC and serum IFN-γ in bronchial asthma group were negatively correlated with FEV1 and PEF (P <0.01). (2) The indexes of lung function in children with bronchial asthma in acute stage and remission stage were significantly different (all P <0.01). (3) Compared with the control group, the levels of total IgE, IFN-γ and serum IL-13 in acute exacerbation and remission stage of bronchial asthma group were significantly different (P all <0.01) . ④Acute exacerbation and remission of IL-13, total lgE and IFN-γin children with bronchial asthma were negatively correlated. ④ The levels of IL-13 and total IgE in children with bronchial asthma were positively correlated with FEV1, FEV1 and FVC and PEF (P <0.01). Conclusions Serum levels of IL-13, IFN-γ and total IgE correlate with the course of asthma and participate in the pathophysiological process, which can be used as an objective indicator of the severity of the disease.