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目的探讨亚急性汞暴露对小鼠肾汞含量及肾转运体表达的影响。方法选取成年昆明种小鼠35只,随机分成5组,每组7只,分别用蒸馏水、氯化汞(32 mg/kg)、甲基汞(2.6 mg/kg)、朱砂(300 mg/kg)和安宫牛黄丸(3 000 mg/kg)灌胃给药,1天1次,44 d后收集肾组织,采用原子荧光分光光度计检测肾汞含量,用RT-PCR法检测肾转运体mRNA的表达。结果与空白组比较,氯化汞组的肾汞蓄积含量是空白组的300倍,甲基汞组是空白组的40倍,而朱砂和安宫牛黄丸组没有明显变化。进一步研究发现:氯化汞诱导有机阴离子转运体Oat1增加6倍,Oat3增加60倍,Oatp4c1增加8倍;诱导外排转运体Mdr1b增加6倍,Mate2-k增加11倍;抑制转运体Oat2降低95%,Oatp1a降低85%,而Urat1增加10倍。甲基汞诱导转运体Oat3增加17倍,Mate2-k增加10倍,Urat1增加7倍,抑制转运体Oat2降低50%,Mrp6降低60%。朱砂诱导Oat3增加20倍。结论氯化汞、甲基汞、朱砂和安宫牛黄丸肾汞蓄积程度不同,其主要原因是对肾转运体的影响不同。
Objective To investigate the effects of subacute mercury exposure on the contents of mercury in kidney and the expression of renal transporter in mice. Methods Thirty - five adult Kunming mice were randomly divided into 5 groups with 7 mice in each group. The mice were treated with distilled water, mercuric chloride (32 mg / kg), methylmercury (2.6 mg / kg) ) And Angong Niuhuang Wan (3000 mg / kg) were administered orally once a day for 44 days. Renal tissues were collected and the content of mercury in the kidney was measured by atomic fluorescence spectrophotometer. The renal transporter mRNA expression. Results Compared with the blank group, the content of renal mercury in mercuric chloride group was 300 times of that in blank group, 40 times in methylmercury group, and no significant change in cinnabar and Angong Niuhuang Wan group. Further studies showed that: Mercuric chloride induced a 6-fold increase in Oat1, a 60-fold increase in Oat3 and a 8-fold increase in Oatp4c1; a 6-fold increase in Mdr1b and a 11-fold increase in Mate2-k; a decrease in Oat2 by 95 %, Oatp1a decreased by 85%, while Urat1 increased by 10 times. Methylmercury-induced transporters Oat3 increased 17-fold, Mate2-k increased 10-fold, Urat1 increased 7-fold, inhibit the transporter Oat2 reduced by 50%, Mrp6 decreased by 60%. Cinnabar induced Oat3 increased 20-fold. Conclusions Mercury chloride, methylmercury, cinnabar and Angong Niuhuang Wan have different levels of renal mercury accumulation, mainly due to different effects on renal transporters.