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目的:观察海人藻酸(Kainic acid,KA)海马内注射后星形胶质细胞的变化及雷公藤甲素(TRP)对其的影响。方法:90只SD大鼠(200～220g)随机分为3组:右侧海马注射生理盐水后生理盐水灌胃作为对照组(NS+NS),右侧海马注射海人藻酸后生理盐水灌胃干预组(KA+NS),右侧海马注射海人藻酸后雷公藤甲素灌胃干预组(KA+TRP)。动物存活1天,3天,5天,7天,14天后免疫组织化学结合图像分析技术观察海马内星形胶质细胞形态和数目的变化。结果:(KA+NS)组海马内星形胶质细胞数目明显增多,胞体明显增大,突起变短,变粗,与(NS+NS)组相比差别具有显著性(p<0.05)(;KA+TRP)组星形胶质细胞数量明显减少,胞体变小,突起变细长,与(KA+NS)组相比差别具有显著性(P<0.05)。结论:KA注射后可导致大鼠海马内星形胶质细胞的激活,雷公藤甲素对KA诱导的星形胶质细胞的活化有抑制作用。
OBJECTIVE: To observe the changes of astrocytes after hippocampal injection of Kaiinic acid (KA) and the effects of triptolide (TRP) on it. Methods: 90 Sprague-Dawley rats (200-220g) were randomly divided into 3 groups: normal saline was administered into the right hippocampus and normal saline was given as a control group (NS+NS). The right hippocampus was injected with kainic acid and physiological saline was given. In the gastric intervention group (KA+NS), the right hippocampus was injected with kainic acid and the triptolide-administered group (KA+TRP). Animals survived for 1 day, 3 days, 5 days, 7 days, and 14 days after immunohistochemistry combined with image analysis techniques to observe changes in the morphology and number of astrocytes in the hippocampus. RESULTS: The number of astrocytes in the hippocampus of the KA+NS group was significantly increased, the cell body was significantly enlarged, the processes became shorter and thicker, and the difference was significant (p<0.05) compared with the (NS+NS) group (p<0.05). The number of astrocytes in the KA+TRP group was significantly decreased, and the cell bodies became smaller and the processes became slender, which was significantly different from that in the KA+NS group (P<0.05). CONCLUSION: KA injection can induce the activation of astrocytes in hippocampus of rats, and triptolide can inhibit the activation of astrocytes induced by KA.