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研究脑内交感抑制系统中中缝隐核（ＮＲＯ）至中脑导水管周围灰质腹侧部（ｖＰＡＧ）多突触通路的儿茶酚胺中间神经元定位。方法在戊巴比妥钠麻醉的大鼠，应用原位杂交组织化学法检测电刺激激活ＮＲＯ对酪氨酸羟化酶（ＴＨ）ｍＲＮＡ表达的影响，同时采用辣根过氧化物酶（ＨＲＰ）逆行标记与免疫组化双标法以观察投射至ｖＰＡＧ的脑干儿茶酚胺能神经元分布。结果激活ＮＲＯ１８～２０ｈ后引起脑干延髓头端腹外侧区（ｒＶＬＭ）、孤束核（ＮＴＳ）、蓝斑（ＬＣ）、中缝背核的ＴＨｍＲＮＡ的表达增加；ＨＲＰ／ＴＨ双标细胞分布于ｒＶＬＭ（Ｃ１）、ＮＴＳ、ＬＣ、黑质网带、室周灰质等区。结论ＮＲＯ至ｖＰＡＧ途径的肾上腺素、去甲肾上腺素神经元主要位于ｒＶＬＭ的Ｃ１区、ＮＴＳ、ＬＣ。
To investigate the localization of catecholamine interneurons in the synaptic inhibitory system from the synaptic midbrain (NRO) to the synaptic duct of the gray matter ventral part of the midbrain (vPAG). Methods In situ hybridization histochemistry was used to detect the effect of NRO on the mRNA expression of tyrosine hydroxylase (TH) in pentobarbital anesthetized rats. The effects of horseradish peroxidase (HRP) Retrograde labeling and double immunohistochemistry to observe the distribution of catecholaminergic neurons in brainstem of vPAG. Results The expression of THmRNA in rVLM, NTS, dorsal raphe nucleus and rostral nucleus of Nodal origin increased from 18 to 20 hours after activation of NRO. HRP / TH double-labeled cells distributed in rVLM (C1), NTS, LC, substantia nigra mesh, ventricular gray matter and other areas. CONCLUSIONS: The adrenergic and norepinephrine neurons from NRO to vPAG pathway are mainly located in the C1 region of rVLM, NTS and LC.