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在哺乳动物中,生物节律与生殖生理和生殖内分泌密切相关,流行病学研究显示长期倒时差的女性表现出生殖力低下和不良妊娠的概率更高,但是人们对生物节律影响生殖的机制了解还很少。本研究旨在以时钟基因Rev-erb-α敲除型(Rev-erb-α-/-)雌性小鼠为模型初步探索生物节律对生殖的影响。将Rev-erb-α-/-和野生型(Rev-erb-α+/+)小鼠与C57BL/6J小鼠交配观察生殖力变化,用苏木精-伊红(HE)染色法观察卵巢组织变化,将Rev-erb-α+/+与Rev-erb-α-/-雌性小鼠分别与C57BL/6J雄性小鼠交配后比较见栓后1.5d统计的胚胎数量,用阴道涂片结合周期图和周期谱观察小鼠生理周期变化。结果显示,Rev-erb-α-/-雌性小鼠与C57BL/6J雄鼠交配是不生育的,并且Rev-erb-α-/-雌性小鼠生成的胚胎数量明显减少。Rev-erb-α-/-雌性小鼠卵巢存在各级卵泡,并且结构与野生型(Rev-erb-α+/+)雌性小鼠相比无明显差异。Rev-erb-α-/-雌性小鼠的生理周期较Rev-erb-α+/+小鼠周期长约3.22d,较Rev-erb-α+/+雌性小鼠延长63.5%。以上结果提示,生物钟基因Rev-erb-α缺失导致雌性小鼠生理周期明显延长,可能是影响雌性小鼠生育的原因之一。因此,生物节律的稳定对于生理周期具有重要作用。
In mammals, circadian rhythms are closely linked to reproductive physiology and reproductive endocrinology. Epidemiological studies have shown that women with long-term fallopian tubes have a higher probability of having poor fertility and poor pregnancy, but people are aware of the mechanisms by which circadian rhythms affect reproduction Few. The aim of this study was to explore the effects of circadian rhythms on reproduction using Rev-erb-alpha knockout (Rev-erb-alpha - / -) female mice as a model. The fecundity was observed by mating with Rev-erb-α - / - and wild-type (Rev-erb-α + / +) mice and C57BL / 6J mice and the ovaries were observed by hematoxylin and eosin Tissue changes were compared between Rev-erb-α + / + and Rev-erb-α - / - female mice and C57BL / 6J male mice respectively. Periodic and Periodic Spectrum Changes in Physiological Cycle in Mice. The results showed that mating of Rev-erb-alpha - / - female mice with C57BL / 6J males was not fertile and the number of embryos produced by Rev-erb-alpha - / - female mice was significantly reduced. There were all levels of follicles in Rev-erb-α - / - female mice ovaries, and there was no significant difference between the structure and wild type (Rev-erb-α + / +) female mice. The physiological cycle of Rev-erb-α - / - female mice was 3.22 days longer than that of Rev-erb-α + / + mice and 63.5% longer than that of Rev-erb-α + / + mice. The above results suggest that the loss of Rev-erb-alpha in the circadian clock gene leads to a significant prolonged physiological cycle in female mice, which may be one of the reasons that affect the reproductive performance of female mice. Therefore, the stabilization of circadian rhythm plays an important role in the physiological cycle.