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目的采用RNA干扰(RNA interference,RNAi)技术敲除胃癌细胞核因子-kappa B(nucle-ar factor-B,NF-B)亚单位p65后,观察其对细胞增殖和侵袭力的影响。方法采用p65小干扰RNA(small interfering RNA,siRNA)转染胃癌细胞后,采用Western Blot检测细胞p65蛋白,分别采用软琼脂集落培养试验和Ttanswell检测癌细胞增殖和侵袭能力。结果 siRNA转染组细胞p65蛋白明显被抑制,且呈浓度依赖性。与对照组比较,转染组细胞所形成的软琼脂集落数和穿膜细胞数明显减少,且呈时间和浓度依赖性。结论针对NF-B重要亚单位的p65siRNA可抑制胃癌细胞侵袭。研究发现,NF-B途径的激活是胃癌等肿瘤细胞生存的重要机制之一。
OBJECTIVE: To knock down the nuclear factor-kappa B (NF-B) subunit p65 of gastric cancer cells by RNA interference (RNAi) technique and observe its effect on cell proliferation and invasiveness. Methods After transfection of p65 small interfering RNA (siRNA) into gastric cancer cells, p65 protein was detected by Western Blot. The proliferation and invasion ability of cancer cells were detected by soft agar colony culture and Ttanswell respectively. Results The p65 protein of siRNA transfection group was significantly inhibited in a concentration-dependent manner. Compared with the control group, the number of soft agar colonies and the number of transmembrane cells formed in transfected cells were significantly decreased in a time and concentration dependent manner. Conclusion The p65siRNA directed against NF-B major subunit can inhibit gastric cancer cell invasion. The study found that activation of NF-B pathway is an important mechanism of survival of tumor cells such as gastric cancer.