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利用高浓度尿素变性蛋白质抑制RNA酶,氯化锂(LiCl)选择性沉淀RNA,酚/氯仿抽提除去变性的蛋白质、脂类及DNA。结果表明:该法适合大量样品少量组织细胞的RNA提取。
High concentrations of urea-denatured protein are used to inhibit RNase, lithium chloride (LiCl) is used to selectively precipitate RNA, phenol / chloroform extraction to remove denatured proteins, lipids and DNA. The results show that this method is suitable for RNA extraction of a small amount of tissue cells in a large number of samples.