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目的:通过检查呼吸窘迫综合症患儿外周血单个核细胞中CD24和TNF-α、IL-6、和IL-17A炎症因子mRNA的表达,探讨其对呼吸窘迫综合症的诊断和预后价值。方法:选择2015年1月至12月在我院接受治疗的32例非感染型呼吸窘迫综合症患儿为研究组,选择同期的30例健康新生患儿为对照组,采集研究组治疗前后和对照组的外周血,分离单个核细胞,采用RT-PCR检测CD24和TNF-α、IL-6、和IL-17A炎症因子mRNA的表达水平。结果:研究组治疗前CD24mRNA表达水平明显高于对照组,差异有统计学意义(P<0.01),而TNF-α、IL-6、和IL-17A mRNA表达水平比较,差异无统计学意义(P>0.05)。研究组治疗后CD24mRNA表达水平明显低于治疗前,差异有统计学意义(P<0.01),而TNF-α、IL-6、和IL-17A mRNA表达水平比较,差异无统计学意义(P>0.05)。结论:呼吸窘迫综合症患儿外周血单个核细胞中CD24mRNA表达水平升高,可能是其诊断和预后的分子标记物。
OBJECTIVE: To investigate the expression of CD24, TNF-α, IL-6 and IL-17A mRNA in peripheral blood mononuclear cells of children with respiratory distress syndrome (ARD) and to explore their diagnostic and prognostic value in the treatment of respiratory distress syndrome. Methods: Thirty-two children with non-infectious respiratory distress syndrome who were treated in our hospital from January to December 2015 were selected as study group and 30 healthy newborn children were selected as control group. The mononuclear cells were isolated from the peripheral blood of the control group. The expression of CD24, TNF-α, IL-6 and IL-17A mRNA was detected by RT-PCR. Results: The expression level of CD24mRNA in the study group before treatment was significantly higher than that in the control group (P <0.01), while there was no significant difference in the expression levels of TNF-α, IL-6 and IL-17A P> 0.05). The expression level of CD24mRNA in the study group was significantly lower than that before treatment (P <0.01), while the levels of TNF-α, IL-6 and IL-17A mRNA in the study group were not significantly different (P> 0.05). Conclusion: The expression of CD24mRNA in peripheral blood mononuclear cells of children with respiratory distress syndrome may be a molecular marker for diagnosis and prognosis.