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目的 研究肝癌肝静脉阻断后肝脏及远隔器官组织血管内皮生长因子受体 1及受体 2的表达 ,探讨肝静脉阻断对肝癌生长及侵袭、转移的影响。方法 以 Wistar大白鼠肝癌模型为材料 ,采用 SUPERVISION免疫组化方法观察肝癌肝静脉阻断后肝癌、癌周、临近肝叶 (肝左中叶 )及肺组织血管内皮生长因子受体 1(Flt- 1)及血管内皮生长国受体 2 (Flk- 1/ KDR)的表达变化。结果 肝静脉阻断后肝癌、癌周组织 Flt- 1的表达较对照组明显下调 (P<0 .0 1) ,肝左叶叶 Flt- 1的表达较对照组明显上调 (P<0 .0 1) ,肝组织 Flt- 1的表达较对照组亦明显上调 (P<0 .0 5 )。肝癌组织 Flk- 1KDR的表达较对照组明显上调 (P<0 .0 5 ) ,癌周组织Flk- 1KDR的阳性表达例数 (占 6 8.7% )多于阴性表达例数 ,但与对照组比较无差异 (P>0 .0 5 ) ,肝左叶叶 Flk- 1KDR的表达较对照组明显上调 (P<0 .0 1) ,肺组织 Flk- 1KDR的表达与对照组间未见差异 (P>0 .0 5 )。对照组各部位 Flt- 1与 Flk- 1KDR的表达未见差异 (P>0 .0 5 )。结论 肝静脉阻断使局部肿瘤坏死 ,同时可能导致癌细胞的扩散。
Objective To study the expression of vascular endothelial growth factor receptor 1 and receptor 2 in liver and distant organ after hepatic vein hepatic vein occlusion and to investigate the influence of hepatic vein occlusion on the growth, invasion and metastasis of hepatocellular carcinoma. Methods The Wistar rat model of hepatocellular carcinoma was used to observe the expression of Flt-1 in hepatocellular carcinoma (HCC), perivenous lobe (adjacent hepatic lobe) and lung tissue in the hepatic vein with SUPERVISION immunohistochemical method. ) And vascular endothelial growth factor receptor 2 (Flk-1 / KDR). Results After the hepatic vein occlusion, the expression of Flt-1 in hepatocellular carcinoma and pericancerous tissues was significantly lower than that in the control group (P <0.01), and the expression of Flt-1 in the left hepatic lobe was significantly higher than that in the control group (P <0. 0 1). The expression of Flt-1 in liver tissue was also significantly up-regulated compared with the control group (P <0.05). The expression of Flk-1KDR in hepatocellular carcinoma was significantly higher than that in control group (P <0.05), and the positive expression rate of Flk-1KDR in cancerous tissue was more than that in negative control group There was no difference (P> 0.05). The expression of Flk-1KDR in the left lobe of the liver was significantly up-regulated compared with the control group (P <0.01). There was no difference between the expression of Flk- > 0 .0 5). There was no difference in the expression of Flt-1 and Flk-1KDR between different groups (P> 0.05). Conclusion Hepatic vein occlusion may cause local tumor necrosis and may lead to the spread of cancer cells.