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目的：前瞻性观察妊娠各期单纯疱疹病毒（ＨＳＶ）垂直传播。方法：用ＥＬＩＳＡ法检测孕妇外周血及相应脐血ＨＳＶＩｇＭ抗体；用ＰＣＲ技术检测宫颈分泌物，异常生育孕妇外周血及相应流产物ＨＳＶＤＮＡ。结果：妊娠早、中、晚各期及脐血ＨＳＶＩｇＭ阳性率分别为１１．９７％、６．３８％、０、８．２０％，ＩｇＭ阳性母亲无垂直传播。宫颈ＨＳＶＤＮＡ阳性率为２．０５％，经宫颈传播率为６６．６７％。异常生育孕妇外周血及相应流产物ＨＳＶＤＮＡ阳性率为２．０２％，２．６１％，经血传播率为５０．００％。感染组死胎畸形及先天感染发生的相对危险度分别为非感染组的６．６倍和２．２倍。二组婴儿体检与智测值无明显差异。结论：ＨＳＶ经宫颈传播大于经血传播，剖宫产不能阻断垂直传播。
Objective: To prospectively observe the vertical transmission of herpes simplex virus (HSV) during pregnancy. Methods: The serum levels of HSVIgM in peripheral blood and corresponding umbilical cord blood of pregnant women were detected by ELISA. The HSVDNA of peripheral blood and corresponding flow products of cervical secretions and abnormal pregnant women were detected by PCR. Results: The positive rates of HSVIgM in early, middle and late pregnancy and umbilical cord blood were 11.97%, 6.38%, 0,8.20%, respectively. There was no vertical transmission of IgM positive mothers. Cervical HSVDNA positive rate was 2.05%, cervical transmission rate was 66.67%. The positive rates of HSVDNA in peripheral blood of pregnant women with abnormal fertility and corresponding abortion products were 2.02% and 2.61% respectively, and the rate of blood transmission was 50.00%. The relative risk of stillbirth and congenital infection in infected group were 6.6 and 2.2 times higher than that in non-infected group respectively. There was no significant difference between the two groups in physical examination and IQ. Conclusion: HSV is more transmitted through the cervix than menstrual blood, and cesarean section can not block the vertical transmission.