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采用RAPD和GISH技术对栉孔扇贝(♀)和虾夷扇贝(♂)杂交子代胚后发育4个重要时期(担轮幼虫期、D形幼虫期、壳顶幼虫期和眼点幼虫期)的遗传构成进行了检测。在RAPD检测中,50条随机引物在亲贝中共扩增出35条栉孔扇贝的特异条带和28条虾夷扇贝特异条带,其中栉孔扇贝特异条带在杂交子代4个时期出现的条数分别为:担轮幼虫期21条、D形幼虫期19条、壳顶幼虫期23条和眼点幼虫期23条;而虾夷扇贝特异条带在杂交子代4个时期出现的条数分别为:17、16、1和1。GISH结果表明,杂交扇贝在担轮幼虫期和D形幼虫期均继承了来自父母本遗传物质,而在壳顶幼虫期和眼点幼虫期未检测到来自父本的遗传物质。结果表明,杂交子代遗传结构在进入壳顶幼虫期时发生重大改变,大部分父本遗传物质从杂交贝基因组中丧失。
Using RAPD and GISH techniques, four important stages (larval stage, D larva stage, shell top larva stage, and eye larval stage) were developed after hybridization between the scallop (♀) and the Japanese scallop (♂) The genetic makeup was tested. In the RAPD assay, 35 random primers were used to amplify 35 specific bands of Chlamys farreri and 28 specific bands of Scallop and Sciardia japonica, and the specific bands of Chlamys farreri appeared in four stages of hybrid progenies Were 21 for larvae, 19 for D larvae, 23 for shell-top larvae and 23 for larvae. However, the occurrence of specific bands of Eriobotrya japonica in four stages of hybrid progeny The numbers are: 17, 16, 1 and 1 respectively. GISH results showed that hybrid scallop inherited genetic material from parents in both larvae and D larvae, while no genetic material from paternal instars was detected in both shell larvae and eyed larvae. The results showed that the genetic structure of hybrid progeny changed significantly when entering the shell top larval stage, and most of the paternal genetic material was lost from the hybrid shellfish genome.