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目的对中药复方热毒宁注射液的解热抗炎活性成分进行研究。方法采用脂多糖(LPS)诱导小鼠内毒素休克模型对热毒宁注射液解热抗炎活性部位进行筛选,综合应用HP-20大孔树脂、硅胶、MCI、ODS和Sephadex LH-20柱色谱以及反相MPLC、HPLC等各种现代色谱技术对解热抗炎活性部位进行系统地分离纯化,根据化合物的光谱数据和理化性质进行结构鉴定。结果经活性筛选,热毒宁注射液经大孔吸附树脂95%乙醇洗脱部位为解热抗炎活性部位,从中分离鉴定了14个化合物,分别鉴定为邻苯二甲酸二丁酯(1)、异香草酸(2)、香草乙酮(3)、苯丙酸(4)、京尼平苷(5)、jasmigeniposide B(6)、京尼平苷酸(7)、京尼平龙胆二糖苷(8)、6″-O-反-对香豆酰基京尼平龙胆二糖(9)、6″-O-反-阿魏酰基京尼平龙胆二糖苷(10)、6″-O-反-芥子酰基京尼平龙胆二糖苷(11)、jasmigeniposide A(12)、6″-O-反-桂皮酰基京尼平龙胆二糖苷(13)、2′-O-反-咖啡酰基栀子新苷(14)。结论化合物1~4、13均为首次从热毒宁注射液中分离得到;经UPLC定性分析及文献对照,化合物5、7~13均为来源于栀子中的化学成分。
OBJECTIVE To study the antipyretic and anti-inflammatory components of traditional Chinese medicine compound Radixin injection. Methods Lipopolysaccharide (LPS) -induced murine model of endotoxic shock was used to screen the antipyretic and anti-inflammatory active sites of RT-injection. HP-20 macroporous resin, silica gel, MCI, ODS and Sephadex LH-20 column chromatography As well as reverse phase MPLC, HPLC and other modern chromatographic techniques for antipyretic anti-inflammatory active site separation and purification, based on the spectral data and physico-chemical properties of compounds for structural identification. Results After the active screening, the injection of Reverining injection through 95% ethanol of macroporous resin as antipyretic and anti-inflammatory active site, from which 14 compounds were identified and identified as dibutyl phthalate (1) , Vanillic acid (2), vanillin (3), phenylpropanoic acid (4), geniposide (5), jasmigeniposide B (6), geniposidic acid (9), 6 “-O-trans-feruloyl genipin gentiobioside (10), 6” -O-trans-p-coumaryl genipin gentiobiose (11), jasmigeniposide A (12), 6 "-O-trans-cinnamoyl genipin gentiobioside (13), 2’-O- - Caffeoyl Gardenoside (14). CONCLUSION Compounds 1 to 4 and 13 are isolated from Revedin injection for the first time. The chemical constituents from Fructus Gardeniae 5 and 13 to 13 were identified by UPLC qualitative analysis and literature control.