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大豆种皮色在从野生大豆到栽培大豆的选择过程中逐渐由黑色变成黄色,是重要的形态标记,因此,大豆种皮色相关基因的研究无论是对进化理论研究还是育种实践都具有非常重要的意义。利用褐色种皮J1265-2大豆及其衍生亲本黄色种皮大豆J1265-1为材料,通过SSR引物扩增片段,检验遗传背景的异同,同时对控制种皮的候选基因GmF3’H进行扩增和测序分析。结果表明,褐色种皮和黄色种皮材料不仅用161对SSR分子标记检测没有发现差异,其褐色种皮候选基因GmF3’H的编码区及起始密码子上游1465 bp序列也是一致的。因此,证明褐色种皮J1265-2大豆与其衍生亲本黄色种皮大豆J1265-1为近等基因系,其控制褐色种皮的基因型与已报道的基因型不同。
Soybean seed coat color is an important morphological marker gradually changing from black to yellow during the process of selection from wild soybean to cultivated soybean. Therefore, the study of soybean seed coat color-related genes is very important both for evolutionary theory research and breeding practice Significance. Using the brown seed coat J1265-2 soybean and its derivative parent yellow seed coat soybean J1265-1 as materials, the similarities and differences of the genetic background were tested by SSR primer amplification, and the candidate gene GmF3’H which controls the seed coat was amplified. Sequencing analysis. The results showed that there was no difference between brown seed coat and yellow seed coat in 161 pairs of SSR markers. The coding region of brown seed coat gene GmF3’H was also consistent with the 1465 bp upstream of start codon. Therefore, it was proved that the brown seed coat J1265-2 soybean and its derived parent yellow seed coat soybean J1265-1 are near-isogenic lines, and the genotype of the brown seed coat is different from the reported genotype.