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定量研究正常和放射复合伤口中VEGF基因表达及其在血管再生中的作用。方法 4 8只wistar二级大鼠背部皮肤制作圆形伤口后以 2 5Gy60 Coγ射线局部照射 ,于伤后 2、5、10、15、2l和 2 8d活杀取材 ,采用免疫组化和图像分析技术研究VEGF基因表达及其意义。结果单创组于伤后 2d ,新生血管内皮细胞浆内VEGF呈强阳性 ;5d时毛细血管数明显增多 ,其内皮细胞浆内VEGF阳性 ;而于 10d后 ,VEGF逐渐减少。伤照组则于伤后 5d呈弱阳性 ,10d时阳性 ,15d后呈弱阳性。定量结果表明 :单创组VEGF于伤后 2 - 10d平均光密度 (MOD)逐渐减少 (P <0 .0 5或P <0 .0 1) ,积分光密度 (IOD)逐渐增加 (P <0 .0 5或P <0 .0 1)。 15d后其MOD和IOD均明显减少。伤照组VEGF的MOD和IOD(P <0 .0 1)于伤后 10d达高峰。照射组与单创组比 ,伤后 5- 10d ,其MOD和IOD均明显减少 (P <0 .0 5或P <0 .0 1) ;伤后 15d ,其IOD较单创组为多 (P <0 .0 5)。结论内源性VEGF基因表达参与创伤愈合中血管再生过程 ;辐射使血管内皮细胞表达VEGF减少 ,强度减弱 ,时间滞后
Quantitative study of VEGF gene expression in normal and radiation-combined wounds and its role in angiogenesis. METHODS: Eighty wistar rats were sacrificed at 2, 5, Gy 60 Coγ-ray irradiation on the skin of the back of the rats. The animals were sacrificed at 2, 5, 10, 15, 21 and 28 days after injury. Immunohistochemistry and image analysis Technical study of VEGF gene expression and its significance. Results In the single wound group, the VEGF in the neovascular endothelial cells was strongly positive 2 days after injury and the number of capillary vessels increased significantly on the 5th day, and the VEGF in the endothelial cells was positive. At 10 days, VEGF decreased gradually. The injury group was weakly positive 5d after injury, positive 10d, weakly positive 15d after injury. The quantitative results showed that the mean optical density (MOD) of the single group decreased gradually from 2 to 10 days after injury (P <0.05 or P <0.01), and the integral optical density (IOD) increased gradually (P <0 .0 5 or P <0 .0 1). After 15d the MOD and IOD were significantly reduced. The MOD and IOD (P <0.01) of VEGF in injury group reached its peak on the 10th day after injury. Compared with the single group, the MOD and IOD of the irradiated group were significantly decreased (P <0.05 or P <0.01) after 5 to 10 days of injury, while the IOD of the irradiated group was more than that of the single group P <0. 05). Conclusions Endogenous VEGF gene expression is involved in the process of angiogenesis in wound healing. Radiation can decrease the expression of VEGF in vascular endothelial cells, decrease the intensity, and lag time