本文作者依据乙型肝炎病毒S基因的核苷酸顺序,用Merrifield固相法合成了代表HBsAg不同区域的几种多肽,通过免疫动物来研究这些多肽的抗原特异性。实验结果表明,抗P3(氨基酸残基2-16),P6(95-109)和P49(110-137)这3种互不重叠多肽的抗体,都能与HBsAg 5种亚型(aywl、ayw2、ayr、adw2和adr)反应,表明组特异性抗原a至少由3个互不重叠的氨基酸顺序组成。 Based on the nucleotide sequence of the hepatitis B virus S gene, the authors synthesized several polypeptides representing different regions of HBsAg using the Merrifield solid phase method and studied the antigen specificity of these polypeptides by immunizing animals. The results showed that the antibodies against P3 (amino acid residues 2-16), P6 (95-109) and P49 (110-137) which do not overlap with each other could bind to the 5 subtypes of HBsAg (ay1, ayw2 , Ayr, adw2 and adr), indicating that the group-specific antigen a consists of at least three non-overlapping amino acid sequences.