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目的观察系统性红斑狼疮(SLE)患者外周血T淋巴细胞p53基因表达情况及探讨其与DNA甲基化的相关性。方法收集34例SLE患者与23例健康对照者,均取外周血T淋巴细胞,分别用5-甲基胞嘧啶抗体与流式细胞仪检测DNA甲基化状态和反转录-聚合酶链反应(real time RT-PCR)分析p53mRNA表达情况。结果 SLE患者DNA甲基化水平活动期(8.50±1.42)与正常人(11.31±1.34)相比,差异有统计学意义(P<0.01)。SLE患者p53基因表达活动期(1.15±0.33)与正常人(0.81±0.27)相比,差异也有统计学意义(P<0.01)。SLE患者p53基因的表达与狼疮疾病活动指数(SLEDAI)正相关(P<0.01);而与DNA甲基化水平没有明显的相关性(P>0.05)。结论 p53基因在外周血T淋巴细胞中的异常表达与SLE的发病相关,p53基因的异常表达在SLE表观遗传学发病机制的作用有待进一步研究。
Objective To observe the expression of p53 gene in peripheral blood T lymphocytes of patients with systemic lupus erythematosus (SLE) and to explore its relationship with DNA methylation. Methods Twenty-four patients with SLE and 23 healthy controls were recruited. Peripheral blood T lymphocytes were collected. Methylation status and reverse transcription-polymerase chain reaction (PCR) were detected by 5-methylcytosine antibody and flow cytometry respectively. (real time RT-PCR) analysis of p53 mRNA expression. Results The DNA methylation level of active SLE patients (8.50 ± 1.42) was significantly higher than that of normal controls (11.31 ± 1.34) (P <0.01). The difference was statistically significant (P <0.01) between SLE patients with active p53 gene expression (1.15 ± 0.33) and normal subjects (0.81 ± 0.27). The p53 gene expression in patients with SLE was positively correlated with SLEDAI (P <0.01), but not with DNA methylation (P> 0.05). Conclusion The abnormal expression of p53 gene in peripheral blood T lymphocytes is associated with the pathogenesis of SLE. The role of p53 gene abnormality in the pathogenesis of epileptic SLE remains to be further studied.