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Late Cretaceous-Cenozoic Exhumation History of the L(u
【出 处】
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null
【发表日期】
:
2010年04期
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目的:观察研究SR-BI上C323位点突变后,对介导细胞选择性摄取胆固醇酯及游离胆固醇释放作用的影响,以进一步明确SR-BI上C323位点在SR-BI功能发挥中的作用。方法:1.免疫印记(Western Blot)检测细胞株SR-BI表达情况。2.通过Caveolae Prep,从蛋白水平检测变异细胞株SR-BI的分布情况。2.1用免疫荧光标记细胞株SR-BI,通过共聚焦显微镜观察变异细胞株SR-
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